affinity chromatography

affinity chromatography
Chromatography in which the immobile phase (bed material) has a specific biological affinity for the substance to be separated or isolated, such as the affinity of an antibody for its antigen, or an enzyme for a substrate analogue.

Dictionary of molecular biology. 2004.

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  • Affinity chromatography — is a method of separating biochemical mixtures and based on a highly specific interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Contents 1 Uses 2 Principle 3 Batch and column setup …   Wikipedia

  • affinity chromatography — n chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be… …   Medical dictionary

  • affinity chromatography — A method for purifying specific components in a solution by exploiting their specific binding to known molecule(s). The mixed solution is passed through a column containing a solid medium to which the binding molecule is covalently attached. See …   Glossary of Biotechnology

  • affinity chromatography — giminiškumo chromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su gėriklio ligandais. atitikmenys: angl. affinity chromatography; biospecific affinity… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • affinity chromatography — giminingumo chromatografija statusas T sritis chemija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su sorbento ligandais. atitikmenys: angl. affinity chromatography; biospecific chromatography rus. афинная… …   Chemijos terminų aiškinamasis žodynas

  • affinity chromatography — noun Date: 1970 chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column treated with a substance having a ligand for which the macromolecule has an affinity that causes it to be… …   New Collegiate Dictionary

  • affinity chromatography — noun : chromatography in which a macromolecule (as a protein) is isolated and purified by passing it in solution through a column that has been treated with a substance having a ligand for which the macromolecule has an affinity that causes it to …   Useful english dictionary

  • biospecific affinity chromatography — giminiškumo chromatografija statusas T sritis Standartizacija ir metrologija apibrėžtis Chromatografija, pagrįsta biologiškai aktyvių medžiagų sąveika su gėriklio ligandais. atitikmenys: angl. affinity chromatography; biospecific affinity… …   Penkiakalbis aiškinamasis metrologijos terminų žodynas

  • Chromatography — Pictured is a sophisticated gas chromatography system. This instrument records concentrations of acrylonitrile in the air at various points throughout the chemical laboratory. Chromatography (from Greek χρῶμα chroma color and γράφειν graphein to… …   Wikipedia

  • chromatography — chromatographer, n. chromatographic /kreuh mat euh graf ik, kroh meuh teuh /, adj. chromatographically, adv. /kroh meuh tog reuh fee/, n. Chem. the separation of mixtures into their constituents by preferential adsorption by a solid, as a column… …   Universalium

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